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Method for isolation and selection of temperature-sensitive mutants of herpes simplex virus.

作者信息

Manservigi R

出版信息

Appl Microbiol. 1974 Jun;27(6):1034-40. doi: 10.1128/am.27.6.1034-1040.1974.

DOI:10.1128/am.27.6.1034-1040.1974
PMID:4364609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380204/
Abstract

A method for induction and selection of temperature-sensitive mutants of herpes simplex virus is presented. After the infected cells were treated with 5-bromodeoxyuridine, the virus was extracted by repeated freezing and thawing and cloned in microcultures which were then incubated at permissive temperature until viral plaques appeared. The microcultures were then replicated at nonpermissive temperature. Clones not forming plaques in these latter were further purified and examined for temperature-sensitive characteristics. Viral clones mutated in plaque-forming ability or in yield were obtained and preliminarily characterized.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/d755dc6d39de/applmicro00041-0062-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/a0188d0bf6e0/applmicro00041-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/8b1cc0981b8a/applmicro00041-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/a0047a76795e/applmicro00041-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/d5a3f39b67e7/applmicro00041-0062-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/d755dc6d39de/applmicro00041-0062-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/a0188d0bf6e0/applmicro00041-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/8b1cc0981b8a/applmicro00041-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/a0047a76795e/applmicro00041-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/d5a3f39b67e7/applmicro00041-0062-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fb/380204/d755dc6d39de/applmicro00041-0062-d.jpg

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引用本文的文献

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Proc Natl Acad Sci U S A. 1984 Apr;81(8):2440-3. doi: 10.1073/pnas.81.8.2440.
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Transition from a heterozygous to a homozygous state of a pair of loci in the inverted repeat sequences of the L component of the herpes simplex virus type 1 genome.
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Collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2.1型和2型单纯疱疹病毒温度敏感突变体的协同互补研究

本文引用的文献

1
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
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Conditional lethal mutants of rabbitpox virus. I. Isolation of host cell-dependent and temperature-dependent mutants.
Virology. 1966 Apr;28(4):592-9. doi: 10.1016/0042-6822(66)90244-3.
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Isolation of temperature-sensitive mutants of adenovirus type 5.5型腺病毒温度敏感突变体的分离
J Gen Virol. 1971 May;11(2):95-101. doi: 10.1099/0022-1317-11-2-95.
J Virol. 1978 Sep;27(3):490-504. doi: 10.1128/JVI.27.3.490-504.1978.
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Qualitative complementation test for temperature-sensitive mutants of herpes simplex virus.
J Virol. 1975 Nov;16(5):1131-6. doi: 10.1128/JVI.16.5.1131-1136.1975.
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Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map.1型单纯疱疹病毒的遗传学研究。温度敏感突变体的分离、它们被归入互补群以及导致连锁图谱的重组分析。
J Gen Virol. 1973 Mar;18(3):329-46. doi: 10.1099/0022-1317-18-3-329.
5
Isolation of temperature-sensitive mutants of murine leukemia virus.鼠白血病病毒温度敏感突变体的分离。
Virology. 1972 Jun;48(3):749-56. doi: 10.1016/0042-6822(72)90158-4.
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Temperature-sensitive mutants of herpes simplex virus type 2.2型单纯疱疹病毒的温度敏感突变体
J Gen Virol. 1971 Nov;13(2):373-6. doi: 10.1099/0022-1317-13-2-373.
7
Temperature-sensitive mutants of herpes simplex virus.单纯疱疹病毒的温度敏感突变体。
Virology. 1970 Dec;42(4):1144-6. doi: 10.1016/0042-6822(70)90364-8.
8
Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus.猴病毒40的基因分析。I. 病毒微量滴定和影印培养技术的描述。
Virology. 1970 Aug;41(4):751-60. doi: 10.1016/0042-6822(70)90439-3.
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Curr Top Microbiol Immunol. 1969;48:1-28. doi: 10.1007/978-3-642-46163-7_1.
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J Gen Virol. 1968 May;2(3):357-64. doi: 10.1099/0022-1317-2-3-357.