利尿剂对蟾蜍膀胱上皮细胞中环3',5'-AMP依赖性蛋白激酶的抑制作用。
Inhibition by diuretics of cyclic 3',5'-AMP-dependent protein kinase from toad bladder epithelium.
作者信息
Ferguson D R, Twite B R
出版信息
Br J Pharmacol. 1973 Oct;49(2):288-92. doi: 10.1111/j.1476-5381.1973.tb08374.x.
Abstract
- A cAMP-dependent protein kinase enzyme has been isolated from toad bladder epithelium.2. This enzyme catalyses the phosphorylation of histones; a reaction stimulated by 0.1 muM cAMP.3. Activity of this enzyme in the presence of 0.1 muM cAMP was significantly inhibited by 100 muM mercuderamide, bumetanide and frusemide. A small, though statistically insignificant inhibition was seen with the same concentrations of hydrochlorothiazide and ethacrynic acid.4. The half maximal inhibition was achieved with mercuderamide, 5 muM, and with frusemide and bumetanide 600 muM.
摘要
已从蟾蜍膀胱上皮中分离出一种环磷酸腺苷（cAMP）依赖性蛋白激酶。
这种酶催化组蛋白的磷酸化；该反应受0.1μM cAMP刺激。
在0.1μM cAMP存在的情况下，该酶的活性被100μM汞撤米特、布美他尼和呋塞米显著抑制。相同浓度的氢氯噻嗪和依他尼酸也有轻微抑制作用，不过在统计学上无显著意义。
汞撤米特5μM、呋塞米和布美他尼600μM可达到半数最大抑制。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder.血管加压素、腺苷 - 3',5'-磷酸（环磷酸腺苷）和茶碱对蟾蜍膀胱作用的相似性。
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Active sodium transport by the isolated toad bladder.离体蟾蜍膀胱的主动钠转运
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Effects of frusemide on sodium and water transport by the isolated toad bladder.速尿对离体蟾蜍膀胱钠和水转运的影响。
Br J Pharmacol Chemother. 1966 Sep;27(3):528-31. doi: 10.1111/j.1476-5381.1966.tb01865.x.
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Actions of vasopressin and aldosterone on the toad bladder: inhibition by ethacrynic acid.血管升压素和醛固酮对蟾蜍膀胱的作用：依他尼酸的抑制作用
J Endocrinol. 1969 Mar;43(3):347-57. doi: 10.1677/joe.0.0430347.
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