[Change in ion transport across the epithelium of the frog skin isolated from the body].

The authors carried out measurements of ionic transport through the epithelium on preparations of isolated and nonisolated skin by the method of Ussing and Zerahn (short-circuiting technique). The blood and nervous connection with the body was preserved in some of the skin preparations (nonisolated) during the experiment. For the purpose the two chambers of Ussing were modified in such a way that this connection could not be interrepted becuase of compression of blood vessels and nerves. Comparative measurements showed that isolation of the skin from the body of a frog caused significant reduction of active transport of ions through the skin epithelium. After addition of acethylcholine, eserine or dipterex to the solution of the internal or external surface of the preparation the nonisolated skins revealed statisticaly not proven tendency to greater reactivity.