Eckstein B, Nimrod A
Endocrinology. 1979 Mar;104(3):711-4. doi: 10.1210/endo-104-3-711.
The effects of FSH, hCG, and PRL on the activity of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) of separate ovarian components of hypophysectomized, diethylstilbestrol-treated rats were studied. Enzyme activity was found to reside mainly in granulosa cells. FSH induced an increase in enzyme activity. A preparation of FSH was purified by adsorbing its LH contamination on rat corpora lutea membranes and by further neutralizing LH traces with an antiserum to the beta-subunit of LH. This purified FSH retained the ability to induce a 6-fold increase in specific enzyme activity in granulosa cells of hypophysectomized, diethylstilbestrol-treated rats. Administration of hCG to rats treated with purified FSH, further enhanced 20 alpha-SDH activity in granulosa cells up to 11.5-fold above control. PRL, which is known to inhibit 20 alpha-SDH activity in regressing rat corpora lutea, suppressed the FSH-induced increase in enzyme activity in the granulosa cells.
研究了促卵泡激素（FSH）、人绒毛膜促性腺激素（hCG）和催乳素（PRL）对垂体切除并用己烯雌酚处理的大鼠卵巢不同组分中20α-羟类固醇脱氢酶（20α-SDH）活性的影响。发现酶活性主要存在于颗粒细胞中。FSH可诱导酶活性增加。通过将FSH中的促黄体生成素（LH）污染物吸附在大鼠黄体膜上，并进一步用抗LHβ亚基血清中和LH痕量，对FSH制剂进行了纯化。这种纯化的FSH保留了在垂体切除并用己烯雌酚处理的大鼠颗粒细胞中诱导特异性酶活性增加6倍的能力。给用纯化FSH处理的大鼠注射hCG，可使颗粒细胞中的20α-SDH活性进一步增强，比对照高11.5倍。已知PRL可抑制大鼠退化黄体中的20α-SDH活性，它可抑制FSH诱导的颗粒细胞中酶活性的增加。
