Dimino M J, Berman S K
Endocrinology. 1979 Mar;104(3):780-3. doi: 10.1210/endo-104-3-780.
[4-14C]Cholesterol side chain cleavage, progesterone synthesis, and cytochrome oxidase activities were measured in mitochondria from unincubated and short term incubated large (8-10 mm) follicles isolated from porcine ovaries. Compared to the activity of mitochondria from unincubated follicles, specific [4-14C]cholesterol side chain cleavage activity in mitochondria from follicles incubated with LH (0.05 microgrogram/ml) did not change significantly after 12 h, but increased almost 2-fold after 18 h and 5-fold after 24 h. Also, specific mitochondrial progesterone synthesis activity increased dramatically after incubation of follicles for 24 h with LH. In comparison, mitochondria prepared from follicles incubated without LH showed no significant change in specific [4-14C]cholesterol side chain cleavage or progesterone synthesis activities after 18 h of incubation. While both of these activities increased after incubating follicles 24 h without LH, the values were significantly lower than those observed for preparations from follicles incubated with LH. In contrast to these changes in mitochondrial steroidogenesis, specific cytochrome oxidase activity in mitochondria did not change after incubation of follicles without or with LH. It is concluded that incubation of follicles with LH stimulates the development of mitochondrial steroidogenesis but initially does not affect some components of the respiratory chain.
对从猪卵巢分离出的未孵育及短期孵育的大卵泡（8 - 10毫米）的线粒体进行了[4 - 14C]胆固醇侧链裂解、孕酮合成及细胞色素氧化酶活性的测定。与未孵育卵泡的线粒体活性相比，用促黄体生成素（LH，0.05微克/毫升）孵育的卵泡线粒体中，特异性[4 - 14C]胆固醇侧链裂解活性在12小时后无显著变化，但在18小时后增加近2倍，24小时后增加5倍。同样，用LH孵育卵泡24小时后，线粒体特异性孕酮合成活性显著增加。相比之下，未用LH孵育的卵泡制备的线粒体在孵育18小时后，特异性[4 - 14C]胆固醇侧链裂解或孕酮合成活性无显著变化。虽然在未用LH孵育卵泡24小时后这两种活性均增加，但数值显著低于用LH孵育的卵泡制备物所观察到的值。与线粒体类固醇生成的这些变化相反，无论卵泡有无LH孵育，线粒体中的特异性细胞色素氧化酶活性均无变化。结论是，用LH孵育卵泡可刺激线粒体类固醇生成的发展，但最初并不影响呼吸链的某些成分。
