Ford H C, Lee E, Engel L L
Endocrinology. 1979 Apr;104(4):857-61. doi: 10.1210/endo-104-4-857.
Hydroxylation of testosterone by hepatic microsomes at positions 6 alpha, 6 beta, 7 alpha, 15 alpha, and 16 alpha has been studied in C57BL/6J, 129/J, and A/J mice. Differences in hydroxylase activities between the C57BL/6J and A/J strains and between the C57BL/6J and 129/J strains were investigated using standard genetic breeding protocols. In the C57BL/6J X A/J line, 6 alpha- and 7 alpha-hydroxylase activity appeared to be under polygenic control in both sexes, as did 15 alpha-hydroxylase activity in females. The lack of 16 alpha-hydroxylase activity observed in 129/J females behaved as a recessive, autosomal, single locus, sex-limited trait. In several instances, the level of hydroxylase activity at one position appeared to be affected by the level of hydroxylase activity at a second position; however, no clear-cut pattern was discerned. Over a period of a year, a remarkable cycle in total hydroxylase activity for all mice was observed; the average activity was greatest in December and least in April.
在C57BL/6J、129/J和A/J小鼠中研究了肝微粒体对睾酮在6α、6β、7α、15α和16α位的羟基化作用。使用标准遗传育种方案研究了C57BL/6J和A/J品系之间以及C57BL/6J和129/J品系之间羟化酶活性的差异。在C57BL/6J×A/J品系中，6α-和7α-羟化酶活性在两性中似乎都受多基因控制，雌性中的15α-羟化酶活性也是如此。在129/J雌性小鼠中观察到的缺乏16α-羟化酶活性表现为一种隐性、常染色体、单基因座、性别限制性状。在一些情况下，一个位置的羟化酶活性水平似乎受到第二个位置的羟化酶活性水平的影响；然而，没有发现明确的模式。在一年的时间里，观察到所有小鼠的总羟化酶活性有一个显著的周期；平均活性在12月最高，4月最低。
