Margreth A, Carraro U, Salviati G
Biochem J. 1974 Jun;139(3):509-13. doi: 10.1042/bj1390509.
The protein composition of sarcoplasmic-reticulum vesicles, either unpurified or after fractionation on sucrose gradients, and with or without previous osmotic shock and sonication, was investigated by electrophoresis in acid polyacrylamide gels. The pattern of release of loosely bound proteins is discussed with respect to their localization in the interior of the vesicles.
采用酸性聚丙烯酰胺凝胶电泳法,对未纯化的或经蔗糖梯度分级分离的肌浆网囊泡(无论有无预先进行渗透压休克和超声处理)的蛋白质组成进行了研究。针对松散结合蛋白在囊泡内部的定位,讨论了其释放模式。