[性激素与从大鼠肝脏和肾脏分离出的溶酶体之间的体外相互作用]
[In vitro interaction between sex hormones and lysosomes isolated from rat liver and kidney].
作者信息
Nichoga V D
出版信息
Farmakol Toksikol. 1979 Mar-Apr;42(2):173-7.
With incubation in vitro of the liver and kidneys lysosomes of castrated rats together with 3H-testosterone and 3H-estradiol in the end concentrations of 2.38.10(-11) M (1 microCu) and 2.8.10(-10) M (2.49 microCu), respectively it was found that the lysosomes can bind labelled hormones. The treatment of lysosomes with membrane-toxic agents (90% aqueous acetone, phospholipase C and 0.1% triton X-100) results in the elimination of labelled steroids from them. In the interaction of 3H-testosterone and 3H-estradiol with lysosomes partake both proteins and lipids of the membranes.
将去势大鼠的肝脏和肾脏溶酶体与终浓度分别为2.38×10⁻¹¹ M（1微克）的³H-睾酮和2.8×10⁻¹⁰ M（2.49微克）的³H-雌二醇进行体外孵育，结果发现溶酶体能够结合标记激素。用膜毒性剂（90%丙酮水溶液、磷脂酶C和0.1% Triton X-100）处理溶酶体，会导致标记类固醇从其中消除。在³H-睾酮和³H-雌二醇与溶酶体的相互作用中，膜的蛋白质和脂质均参与其中。
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