Clayton R B, Cooper J M, Curstedt T, Sjövall J, Borsook H, Chin J, Schwarz A
J Lipid Res. 1974 Nov;15(6):557-62.
A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. The biologically active components are sphingosine ceramides of tetracosanoic and dehydrotetracosanoic acids. Tests of a series of related ceramides show a high degree of structural specificity for the C(24)-V-acyl compounds with significant but markedly lower activity of the C(22) analog. Commercially available sphingomyelin shows activity comparable to that of the tetracosanoic acid ceramide. Sphingosine and tetracosanic acid supplied in equimolar amounts have negligible activity. The results, in the context of other findings, suggest a possible supportive role of plasma ceramides and sphingomyelins in red cell maturation.
一种先前从白细胞中分离出来的脂质因子，在体外培养的兔骨髓细胞系统中发现它能刺激嗜碱性成红细胞的形成，现已通过薄层色谱法、气液色谱法和气液色谱 - 质谱分析法进行了分析。其生物活性成分是二十四烷酸和脱氢二十四烷酸的鞘氨醇神经酰胺。一系列相关神经酰胺的测试表明，对于具有显著活性但明显较低活性的C(22)类似物的C(24)-V-酰基化合物具有高度的结构特异性。市售的鞘磷脂显示出与二十四烷酸神经酰胺相当的活性。等摩尔量供应的鞘氨醇和二十四烷酸的活性可忽略不计。结合其他研究结果，这些结果表明血浆神经酰胺和鞘磷脂在红细胞成熟过程中可能起到支持作用。
