Birkedal-Hansen H, Cobb C M, Taylor R E, Fullmer H M
J Oral Pathol. 1974;3(5):232-8. doi: 10.1111/j.1600-0714.1974.tb01716.x.
A collagenase active against native collagen was found in culture fluids of bovine gingiva. The enzyme first appeared in the culture fluid after 1-2 days and could be harvested thereafter for at least 30 days. The collagenase attacked collagen fibrils and cleaved collagen in solution, resulting in reaction products 1/4 and 3/4 of the length of the original molecule. The enzyme was inhibited by serum, by EDTA and by cysteine. The molecular weight was estimated by gel filtration to be 63,000 daltons.
在牛牙龈的培养液中发现了一种对天然胶原蛋白有活性的胶原酶。该酶在培养1-2天后首次出现在培养液中，此后至少30天可进行收获。这种胶原酶能攻击胶原纤维并裂解溶液中的胶原蛋白，产生的反应产物长度为原始分子的1/4和3/4。该酶受到血清、乙二胺四乙酸（EDTA）和半胱氨酸的抑制。通过凝胶过滤法估计其分子量为63,000道尔顿。
