牛牙龈胶原酶：证明与初步特性研究

Bovine gingival collagenase: demonstration and initial characterization.

作者信息

Birkedal-Hansen H, Cobb C M, Taylor R E, Fullmer H M

出版信息

J Oral Pathol. 1974;3(5):232-8. doi: 10.1111/j.1600-0714.1974.tb01716.x.

DOI:10.1111/j.1600-0714.1974.tb01716.x

Abstract

A collagenase active against native collagen was found in culture fluids of bovine gingiva. The enzyme first appeared in the culture fluid after 1-2 days and could be harvested thereafter for at least 30 days. The collagenase attacked collagen fibrils and cleaved collagen in solution, resulting in reaction products 1/4 and 3/4 of the length of the original molecule. The enzyme was inhibited by serum, by EDTA and by cysteine. The molecular weight was estimated by gel filtration to be 63,000 daltons.

摘要

在牛牙龈的培养液中发现了一种对天然胶原蛋白有活性的胶原酶。该酶在培养1-2天后首次出现在培养液中，此后至少30天可进行收获。这种胶原酶能攻击胶原纤维并裂解溶液中的胶原蛋白，产生的反应产物长度为原始分子的1/4和3/4。该酶受到血清、乙二胺四乙酸（EDTA）和半胱氨酸的抑制。通过凝胶过滤法估计其分子量为63,000道尔顿。