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胰高血糖素刺激的灌注大鼠肝脏中的核蛋白激酶活性

Nuclear protein kinase activity in glucagon-stimulated perfused rat livers.

作者信息

Palmer W K, Castagna M, Walsh D A

出版信息

Biochem J. 1974 Nov;143(2):469-71. doi: 10.1042/bj1430469.

DOI:10.1042/bj1430469
PMID:4376948
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168405/
Abstract

Nuclei isolated from glucagon-stimulated perfused rat livers contained 2-3 times as much protein kinase activity as did nuclei from control animals. In the presence of either the heat-stable inhibitor or the protein kinase regulatory subunit the elevated cyclic AMP-independent enzyme activity from stimulated nuclei was inhibited to an activity equivalent to that found in controls.

摘要

从胰高血糖素刺激的灌注大鼠肝脏中分离出的细胞核所含的蛋白激酶活性是对照动物细胞核的2至3倍。在存在热稳定抑制剂或蛋白激酶调节亚基的情况下，受刺激细胞核中升高的不依赖环磷酸腺苷的酶活性被抑制至与对照中发现的活性相当的水平。

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Nuclear protein kinase activity in glucagon-stimulated perfused rat livers.胰高血糖素刺激的灌注大鼠肝脏中的核蛋白激酶活性
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2
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Changes in adenosine 3',5'-monophosphate level and protein kinase activity by glucagon in rat liver nuclei.胰高血糖素对大鼠肝细胞核中3',5'-环磷酸腺苷水平及蛋白激酶活性的影响
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Rouxs Arch Dev Biol. 1986 May;195(4):276-280. doi: 10.1007/BF02438961.
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3
Direct cytochemical localization of catalytic subunits dissociated from cAMP-dependent protein kinase in Reuber H-35 hepatoma cells. II. Temporal and spatial kinetics.Reuber H-35肝癌细胞中与环磷酸腺苷依赖性蛋白激酶解离的催化亚基的直接细胞化学定位。II. 时空动力学。
J Cell Biol. 1982 Jun;93(3):727-34. doi: 10.1083/jcb.93.3.727.
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Specific nuclear binding of adenosine 3',5'-monophosphate-binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage.3',5'-环磷酸腺苷结合蛋白复合物在胚胎鸡软骨中的特异性核结合及随后的多聚腺苷酸RNA合成。
J Clin Invest. 1980 Sep;66(3):532-42. doi: 10.1172/JCI109885.
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J Cell Biol. 1985 Sep;101(3):965-75. doi: 10.1083/jcb.101.3.965.
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Isolation of pure and unaltered liver nuclei morphology and biochemical composition.分离出形态和生化组成纯净且未改变的肝细胞核。
Exp Cell Res. 1956 Aug;11(2):317-21. doi: 10.1016/0014-4827(56)90107-0.
3
Gluconeogenesis in the perfused rat liver.灌注大鼠肝脏中的糖异生作用。
Biochem J. 1966 Nov;101(2):284-92. doi: 10.1042/bj1010284.
4
Phosphorylation of histones in vivo under the control of cyclic AMP and hormones.
Adv Biochem Psychopharmacol. 1970;3:307-23.
5
Protein kinases: aspects of their regulation and diversity.
Recent Prog Horm Res. 1973;29:329-59. doi: 10.1016/b978-0-12-571129-6.50012-9.
6
Characterization of the interaction of a protein inhibitor with adenosine 3',5'-monophosphate-dependent protein kinases. I. Interaction with the catalytic subunit of the protein kinase.一种蛋白抑制剂与3',5'-环磷酸腺苷依赖性蛋白激酶相互作用的表征。I. 与蛋白激酶催化亚基的相互作用。
J Biol Chem. 1972 Oct 25;247(20):6637-42.
7
Multiple forms of hepatic adenosine 3':5'-monophosphate dependent protein kinase.肝脏3':5'-单磷酸腺苷依赖性蛋白激酶的多种形式。
Biochemistry. 1971 Sep 14;10(19):3614-21. doi: 10.1021/bi00795a020.
8
On the mechanism of action of cyclic AMP.
Adv Biochem Psychopharmacol. 1970;3:287-306.
9
Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases.兔骨骼肌3',5'-环磷酸腺苷依赖性蛋白激酶的纯化及性质
J Biol Chem. 1971 Apr 10;246(7):1986-95.
10
Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.克雷布斯·E·G：3',5'-环磷酸腺苷依赖性蛋白激酶的一种蛋白抑制剂的纯化与特性鉴定
J Biol Chem. 1971 Apr 10;246(7):1977-85.