Carels M, Shepherd D
Can J Microbiol. 1979 Dec;25(12):1484-8. doi: 10.1139/m79-231.
Monascus rubiginosus ATCC 16367 was cultivated aerobically in media containing ammonium and nitrate as nitrogen source. The pH of the medium was adjusted at different times, the pH of the nitrate medium being lowered to the pH of the ammonium medium and the pH of the ammonium medium raised to that of the nitrate. More phosphate was taken up on the nitrate medium, but potassium uptake did not start until 24h. On the ammonium medium, both were taken up in parallel from the beginning, but the amount of phosphate taken up never reached the same level as on nitrate medium. When the pH was adjusted, the uptakes changed, especially on the ammonium medium where a great increase in phosphate uptake was observed. More conidia were formed on the nitrate medium and more pigment on the ammonium medium. When the pH of either media was adjusted, the development of conidia and pigment production changed to that of the other control medium where the pH evolved normally in the direction of the change, regardless of the source of nitrogen. The reasons for the development of conidia on nitrate medium or where the pH is high, and the production of pigment on ammonium medium or at low pH is discussed.
将红曲霉ATCC 16367在含有铵盐和硝酸盐作为氮源的培养基中进行好氧培养。在不同时间调节培养基的pH值，将硝酸盐培养基的pH值降低至铵盐培养基的pH值，同时将铵盐培养基的pH值提高至硝酸盐培养基的pH值。在硝酸盐培养基上吸收的磷酸盐更多，但钾的吸收直到24小时后才开始。在铵盐培养基上，两者从一开始就并行吸收，但吸收的磷酸盐量从未达到硝酸盐培养基上的水平。当调节pH值时，吸收情况发生变化，尤其是在铵盐培养基上，观察到磷酸盐吸收大幅增加。在硝酸盐培养基上形成更多分生孢子，在铵盐培养基上产生更多色素。当调节任何一种培养基的pH值时，分生孢子的发育和色素产生会变为另一种对照培养基的情况，其中pH值朝着变化方向正常演变，而与氮源无关。文中讨论了在硝酸盐培养基上或pH值较高时产生分生孢子，以及在铵盐培养基上或低pH值时产生色素的原因。
