Haslett B G, Cammack R
Biochem J. 1974 Dec;144(3):567-72. doi: 10.1042/bj1440567.
The plastocyanin content of etiolated bean leaves (Phaseolus vulgaris L.) was measured, and the development of the protein in response to light was followed. Measurements were made by quantitative extraction of plastocyanin and a sensitive assay with an O(2) electrode. The electron-paramagnetic-resonance (e.p.r.) signal of oxidized plastocyanin was used as an independent check on the validity of the assay method, and on the thoroughness of extraction. After an initial lag period, the amount of plastocyanin in greening bean leaves increased to reach a maximum after 50h illumination. The chlorophyll/plastocyanin ratio reached a maximum value of 200 irrespective of the light intensity at which greening was carried out, suggesting that the synthesis of the two components is co-ordinated. Experiments involving treatment of etiolated seedlings with brief periods of light of different spectral composition indicated that phytochrome is involved in plastocyanin synthesis. The lack of inhibition of plastocyanin synthesis by specific inhibitors of chloroplast protein synthesis suggests that the protein is synthesized on cytoplasmic ribosomes. The data are discussed in relation to the development of ferredoxin in greening bean leaves.
测定了黄化菜豆叶片（菜豆）中的质体蓝素含量，并追踪了该蛋白在光照下的发育情况。通过对质体蓝素进行定量提取并用氧电极进行灵敏测定来进行测量。氧化型质体蓝素的电子顺磁共振（e.p.r.）信号被用作对测定方法有效性以及提取彻底性的独立检验。经过初始的延迟期后，绿化菜豆叶片中的质体蓝素含量在光照50小时后增加至最大值。叶绿素/质体蓝素比值无论在何种光照强度下进行绿化均达到最大值200，这表明这两种成分的合成是协调的。涉及用不同光谱组成的短时光照处理黄化幼苗的实验表明，光敏色素参与质体蓝素的合成。叶绿体蛋白合成的特异性抑制剂对质体蓝素合成缺乏抑制作用，这表明该蛋白是在细胞质核糖体上合成的。结合绿化菜豆叶片中铁氧还蛋白的发育情况对这些数据进行了讨论。
