Department of Plant Physiology, University of Amsterdam, Icapdijk 26, Amsterdam, The Netherlands.
Planta. 1977 Jan;133(3):289-94. doi: 10.1007/BF00380691.
During light-induced greening of 10-dayold etiolated bean seedlings a strong increase is observed of ferredoxin (Fd) and of ferredoxin-NADP-oxidoreductase (FNR; E.C. 1.6.99.4) activity, both known to reside in chloroplasts. The production of Fd starts immediately upon illumination and proceeds almost linearly for at least the next 72 h. It is inhibited by chloramphenicol (CAP) but not by cycloheximide (CHI), beit that in the presence of the latter Fd synthesis was impaired after 48 h of illumination. We conclude that for the elaboration of functional Fd in greening chloroplasts protein synthesis on chloroplast ribosomes is required. The increase of FNR activity shows a lag of about 24 h and is blocked by both CAP and CHI. When CAP is applied at 24 h after the illumination started it has no effect. We suggest that the synthesis of FNR on cytoplasmic ribosomes requires prior synthesis of protein(s) on chloroplast ribosomes.The nature of possible interactions between chloroplasts and cytoplasm is discussed.
在 10 天大的黄化豌豆幼苗的光诱导绿色化过程中，观察到铁氧还蛋白（Fd）和铁氧还蛋白-NADP-氧化还原酶（FNR；EC 1.6.99.4）活性的强烈增加，这两种物质都已知存在于叶绿体中。Fd 的产生在光照后立即开始，并在至少接下来的 72 小时内几乎呈线性进行。它被氯霉素（CAP）抑制，但不受环己酰亚胺（CHI）的抑制，尽管在后者存在的情况下，在光照 48 小时后 Fd 的合成受到损害。我们得出结论，对于在绿色化叶绿体中产生功能性 Fd，需要在叶绿体核糖体上进行蛋白质合成。FNR 活性的增加显示出约 24 小时的滞后，并且被 CAP 和 CHI 阻断。当在光照开始后 24 小时施加 CAP 时，它没有效果。我们建议，细胞质核糖体上 FNR 的合成需要叶绿体核糖体上蛋白质的先前合成。讨论了叶绿体和细胞质之间可能的相互作用的性质。
