Hinners T A, Terrill W J, Kent J L, Colucci A V
Environ Health Perspect. 1974 Aug;8:191-9. doi: 10.1289/ehp.748191.
Extensive ambient extraction of several metals from human hair compromises some assumptions concerning the binding of metals to hair and the biochemical process of metal incorporation into hair. Hair metal concentrations can reveal metal intoxications and metal deficiencies.The scalp hair selected for investigation included specimens of different colors from both sexes for various donor ages and from different geographic exposure areas. The hair samples were washed by agitation for 30 min with a 1% solution of sodium lauryl sulfate in a pH 7.2 buffer, rinsed repeatedly with deionized water, and oven-dried at 110 degrees C before 2-g portions were taken for extraction and digestion. Extraction involved agitation of the washed hair samples for 21 hr with 40 ml of 1% nitric acid at room temperature (23 degrees C). The extracted hair was digested in order to quantitate the amount of metal that was not extracted. Metal measurements were made by conventional flame atomic absorption spectroscopy. Potential interferences were investigated. Extraction varied between 82 and 100% for Na, Mg, K, Ca, Mn, Zn, Cd, and Pb. Removal of Fe, Ni, and Cu was 33-54%. Anatomical location, binding chemistry, biochemical incorporation, and mass screening are discussed.
从人发中大量提取多种金属，这对一些关于金属与头发结合以及金属掺入头发的生化过程的假设提出了挑战。头发中的金属浓度可以揭示金属中毒和金属缺乏情况。所选择用于研究的头皮头发包括来自不同性别、不同供体年龄以及不同地理暴露区域的不同颜色的样本。头发样本先用1%十二烷基硫酸钠溶液在pH 7.2缓冲液中搅拌洗涤30分钟，然后用去离子水反复冲洗，并在110摄氏度下烘干，之后取2克部分用于提取和消化。提取过程是将洗涤后的头发样本在室温（23摄氏度）下与40毫升1%硝酸搅拌21小时。对提取后的头发进行消化，以定量未被提取的金属量。通过传统火焰原子吸收光谱法进行金属测量。研究了潜在干扰。钠、镁、钾、钙、锰、锌、镉和铅的提取率在82%至100%之间。铁、镍和铜的去除率为33%至54%。文中讨论了解剖位置、结合化学、生化掺入和大规模筛查。
