文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Pietruszko R, Baron D N

Biochem J. 1965 Aug;96(2):557-66. doi: 10.1042/bj0960557.

An enzyme from rat liver, catalysing 3alpha-hydroxy steroid-dependent NAD(P) transhydrogenation and NAD-linked and NADP-linked dehydrogenation of 3alpha-hydroxy steroids, has been purified 100-fold by chromatography on DEAE-cellulose and calcium phosphate gel. 2. No separation of these activities into different protein fractions has been achieved. 3. The properties of the enzyme in catalysing NAD-linked and NADP-linked dehydrogenation have been compared, with androsterone as substrate. Differences were found in pH optima, affinity for coenzyme and steroid, equilibrium constants and effects of salts. 4. NAD-linked dehydrogenation is inhibited by NADPH(2) but is protected from this inhibition by chloride, which alone is itself an inhibitor. 5. The relevance of these findings to the problem of the number of enzymes involved in catalysis of 3alpha-hydroxy steroid-dependent transhydrogenation is discussed.

一种来自大鼠肝脏的酶，可催化3α-羟基类固醇依赖性NAD(P)转氢作用以及3α-羟基类固醇的NAD连接和NADP连接的脱氢反应，已通过DEAE-纤维素和磷酸钙凝胶柱层析纯化了100倍。2. 尚未实现将这些活性分离到不同的蛋白质组分中。3. 以雄甾酮为底物，比较了该酶催化NAD连接和NADP连接的脱氢反应的特性。发现pH最适值、对辅酶和类固醇的亲和力、平衡常数以及盐的影响存在差异。4. NAD连接的脱氢反应受到NADPH₂的抑制，但氯化物可保护其免受这种抑制，而氯化物本身就是一种抑制剂。5. 讨论了这些发现与参与3α-羟基类固醇依赖性转氢作用催化的酶数量问题的相关性。

Pietruszko R, Baron D N

Biochem J. 1965 Aug;96(2):557-66. doi: 10.1042/bj0960557.

An enzyme from rat liver, catalysing 3alpha-hydroxy steroid-dependent NAD(P) transhydrogenation and NAD-linked and NADP-linked dehydrogenation of 3alpha-hydroxy steroids, has been purified 100-fold by chromatography on DEAE-cellulose and calcium phosphate gel. 2. No separation of these activities into different protein fractions has been achieved. 3. The properties of the enzyme in catalysing NAD-linked and NADP-linked dehydrogenation have been compared, with androsterone as substrate. Differences were found in pH optima, affinity for coenzyme and steroid, equilibrium constants and effects of salts. 4. NAD-linked dehydrogenation is inhibited by NADPH(2) but is protected from this inhibition by chloride, which alone is itself an inhibitor. 5. The relevance of these findings to the problem of the number of enzymes involved in catalysis of 3alpha-hydroxy steroid-dependent transhydrogenation is discussed.

一种来自大鼠肝脏的酶，可催化3α-羟基类固醇依赖性NAD(P)转氢作用以及3α-羟基类固醇的NAD连接和NADP连接的脱氢反应，已通过DEAE-纤维素和磷酸钙凝胶柱层析纯化了100倍。2. 尚未实现将这些活性分离到不同的蛋白质组分中。3. 以雄甾酮为底物，比较了该酶催化NAD连接和NADP连接的脱氢反应的特性。发现pH最适值、对辅酶和类固醇的亲和力、平衡常数以及盐的影响存在差异。4. NAD连接的脱氢反应受到NADPH₂的抑制，但氯化物可保护其免受这种抑制，而氯化物本身就是一种抑制剂。5. 讨论了这些发现与参与3α-羟基类固醇依赖性转氢作用催化的酶数量问题的相关性。