Southren A L, Gordon G G, Yeh H S, Dunn M W, Weinstein B I
Invest Ophthalmol Vis Sci. 1979 May;18(5):517-21.
The cytoplasmic glucocorticoid receptor of the iris--ciliary body of the rabbit has been shown to translocate to the cell nucleus within 30 min of an injection of cortisol. Over the next 2 1/2 hr the amount of receptor returns to the control value. The threshold of the loss of receptor from the cytosol was found at 0.04 mg of cortisol per kilogram body weight, with a maximal loss being reached at a cortisol dose of 0.5 mg/kg B.W. The inactive glucocorticoid tetrahydrocortisol and the major sex steroids, dihydrotestosterone, estradiol, and progesterone, had little or no effect on this translocation, indicating the specificity of the cortisol effect. Thus this receptor appears to migrate to the cell nucleus in a manner similar to that found in other steroid-sensitive tissues and is consistent with the accepted mechanism whereby these hormones regulate differential gene expression in the nucleus.
已证明，给兔子注射皮质醇后30分钟内，其虹膜睫状体的细胞质糖皮质激素受体会转移至细胞核。在接下来的2个半小时内，受体数量恢复至对照值。发现每千克体重注射0.04毫克皮质醇时，细胞质中的受体开始丢失，皮质醇剂量为0.5毫克/千克体重时，受体丢失达到最大值。无活性的糖皮质激素四氢皮质醇以及主要的性类固醇，如双氢睾酮、雌二醇和孕酮，对这种转移几乎没有影响，这表明皮质醇作用具有特异性。因此，这种受体似乎以与其他类固醇敏感组织中相似的方式迁移至细胞核，这与这些激素调节细胞核中差异基因表达的公认机制相符。
