Pollock T J, Tessman E S, Tessman I
J Bacteriol. 1979 Apr;138(1):122-5. doi: 10.1128/jb.138.1.122-125.1979.
Nonsense mutations in capsid genes F and G of phages S13 and phi X174 decreased the expression of genes downstream. These polar effects were suppressed by ultraviolet irradiation of the host before infection. Activities of the downstream genes were restored to between 30 and 95% of their normal levels, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral proteins. The effect of ultraviolet irradiation may be to suppress rhodependent termination of transcription. The polarity of one mutation was poorly suppressed by ultraviolet irradiation.
噬菌体S13和φX174衣壳基因F和G中的无义突变降低了下游基因的表达。这些极性效应在感染前通过宿主的紫外线照射得以抑制。通过对病毒蛋白进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定,下游基因的活性恢复到其正常水平的30%至95%之间。紫外线照射的作用可能是抑制转录的依赖于ρ因子的终止。紫外线照射对一个突变的极性抑制效果较差。