脆弱拟杆菌中的超氧化物歧化酶与氧气致死性
Superoxide dismutase and O2 lethality in Bacteroides fragilis.
作者信息
Privalle C T, Gregory E M
出版信息
J Bacteriol. 1979 Apr;138(1):139-45. doi: 10.1128/jb.138.1.139-145.1979.
Abstract
Exposure of midlog Bacteroides fragils (VPI 2393) to 2% O2-98% N2 caused a three- to fivefold increase in superoxide dismutase specific activity within the cells. The increase in specific activity was completed within 90 min after exposure to oxygen and was dependent upon protein synthesis. Cells containing the higher superoxide dismutase level were more resistant to the effects of 5 atm of oxygen tension than were cells containing the lower level of superoxide dismutase but were equally resistant to 5 atm of nitrogen tension. Similar results were observed upon comparing viability experiments with B. fragilis and B. vulgatus. Superoxide dismutase activity in sonic extracts of B. fragilis was rapidly inactivated by exposure to 5 mM H2O2 and was inhibited by 1 mM NaN3 but not 5 mM NaCN. The inhibition pattern is identical to the pattern demonstrated for the purified iron-containing enzyme from Escherichia coli B and suggests that the superoxide dismutase in B. fragilis is an iron enzyme.
摘要
对数中期的脆弱拟杆菌（VPI 2393）暴露于2%氧气-98%氮气环境中，导致细胞内超氧化物歧化酶的比活性增加了三到五倍。比活性的增加在暴露于氧气后90分钟内完成，且依赖于蛋白质合成。超氧化物歧化酶水平较高的细胞比超氧化物歧化酶水平较低的细胞对5个大气压的氧张力影响更具抗性，但对5个大气压的氮张力具有同等抗性。在比较脆弱拟杆菌和普通拟杆菌的活力实验时也观察到了类似结果。脆弱拟杆菌的超声提取物中的超氧化物歧化酶活性通过暴露于5 mM过氧化氢而迅速失活，并被1 mM叠氮化钠抑制，但不被5 mM氰化钠抑制。这种抑制模式与从大肠杆菌B中纯化的含铁酶所显示的模式相同，表明脆弱拟杆菌中的超氧化物歧化酶是一种含铁酶。
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