Perry J J, Evans J B
J Bacteriol. 1967 Jun;93(6):1839-46. doi: 10.1128/jb.93.6.1839-1846.1967.
The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated that key enzymes for glucose catabolism were present in M. sodonensis in quantities equivalent to those of E. coli. The glucose-6-phosphate and 6-phosphogluconate dehydrogenases of M. sodonensis were nicotinamide adenine dinucleotide phosphate (NADP) specific, and glyceraldehyde-3-phosphate dehydrogenase was nicotinamide adenine dinucleotide specific. Transhydrogenase and reduced NADP oxidase were absent. Growth of the organism in the presence of glucose did not result in a repressed ability to oxidize tricarboxylic acid cycle intermediates, but these cells did have a decreased capacity for glucose degradation. The addition of substrates rich in growth-promoting substances, e.g., yeast extract, did not provide requisite nutrients for growth on glucose. Studies with (32)P suggest that M. sodonensis is incapable of synthesizing energy-rich phosphate compounds during the catabolism of glucose.
对嗜钠微球菌在以葡萄糖作为唯一碳源和能源时无法生长的情况进行了研究。对葡萄糖分解代谢途径的评估表明,该微生物同时存在糖酵解途径和磷酸己糖途径。与大肠杆菌的比较研究表明,嗜钠微球菌中存在的葡萄糖分解代谢关键酶的量与大肠杆菌相当。嗜钠微球菌的葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶对烟酰胺腺嘌呤二核苷酸磷酸(NADP)具有特异性,而3-磷酸甘油醛脱氢酶对烟酰胺腺嘌呤二核苷酸具有特异性。不存在转氢酶和还原型NADP氧化酶。该微生物在葡萄糖存在的情况下生长,并不会导致其氧化三羧酸循环中间产物的能力受到抑制,但这些细胞的葡萄糖降解能力确实有所下降。添加富含生长促进物质的底物,如酵母提取物,并未为在葡萄糖上生长提供所需的营养物质。用(32)P进行的研究表明,嗜钠微球菌在葡萄糖分解代谢过程中无法合成富含能量的磷酸化合物。