Perry J J, Evans J B
J Bacteriol. 1966 Jan;91(1):33-8. doi: 10.1128/jb.91.1.33-38.1966.
Perry, Jerome J. (North Carolina State University, Raleigh), and James B. Evans. Oxidation and assimilation of carbohydrates by Micrococcus sodonensis. J. Bacteriol. 91:33-38. 1966.-Micrococcus sodonensis is a biotin-requiring strict aerobe that cannot utilize carbohydrates as sole sources of carbon and energy. However, addition of mannose, glucose, sucrose, or maltose to a medium on which the organism can grow resulted in an increase in total growth. M. sodonensis oxidized these sugars without induction, thus indicating the presence of constitutive enzymes for their transport, activation, and metabolism. Under appropriate nonproliferating cell conditions, glucose was readily incorporated into essential constituents of the cell. When glucose-1-C(14) and glucose-6-C(14) were oxidized by nonproliferating cells, the label was found in both the protein and nucleic acid fractions of the cell. The respiratory quotients of cells oxidizing glucose in saline and in phosphate buffer indicated assimilation of sugar carbon in buffer and virtually no assimilation in saline. The ability of M. sodonensis to completely oxidize glucose and to grow on intermediates of glucose oxidation but not on glucose suggests that glucose may suppress or repress some reaction(s) necessary for growth, and that growth substrates either derepress or circumvent this block.
佩里，杰罗姆·J.（北卡罗来纳州立大学，罗利），以及詹姆斯·B.埃文斯。嗜钠微球菌对碳水化合物的氧化与同化作用。《细菌学杂志》91:33 - 38。1966年。——嗜钠微球菌是一种需要生物素的严格需氧菌，不能将碳水化合物作为唯一的碳源和能源。然而，在该生物体能够生长的培养基中添加甘露糖、葡萄糖、蔗糖或麦芽糖会导致总生长量增加。嗜钠微球菌无需诱导就能氧化这些糖类，这表明存在用于其转运、激活和代谢的组成型酶。在适当的非增殖细胞条件下，葡萄糖很容易被整合到细胞的必需成分中。当非增殖细胞氧化葡萄糖 - 1 - C(14)和葡萄糖 - 6 - C(14)时，标记物出现在细胞的蛋白质和核酸组分中。在盐溶液和磷酸盐缓冲液中氧化葡萄糖的细胞的呼吸商表明，缓冲液中的糖碳被同化，而在盐溶液中几乎没有同化作用。嗜钠微球菌能够完全氧化葡萄糖并在葡萄糖氧化的中间产物上生长，但不能在葡萄糖上生长，这表明葡萄糖可能抑制或阻遏生长所需的某些反应，并且生长底物要么解除这种阻遏，要么绕过这个障碍。
