对奥普托欣耐药的肺炎球菌中黄递酶活性的改变。
Altered diaphorase activity in optochin-resistant pneumococci.
作者信息
Kreger A S, Olsen R H, Talmadge M B
出版信息
J Bacteriol. 1968 Oct;96(4):1021-8. doi: 10.1128/jb.96.4.1021-1028.1968.
Methylene blue (MB) reductase activities of washed cell suspensions and of cell-free extracts prepared from optochin-resistant mutant pneumococci were two times greater than and four to eight times more resistant to optochin inhibition than those of similar preparations from the optochin-sensitive parent strains. With whole cells, optochin hydrochloride was approximately four times more potent than quinine hydrochloride in inhibiting MB reductase activity. However, with cell-free extracts, both drugs had similar inhibitory activities. Glucose uptake was not affected by optochin hydrochloride, and both optochin-sensitive and optochin-resistant pneumococci had similar glucose uptake patterns. Diaphorase activities of cell-free extracts prepared from optochin-resistant pneumococci were two times greater than and four to eight times more resistant to optochin inhibition than those of cell-free extracts prepared from the optochin-sensitive parent strains. Flavin concentrations of cell-free extracts prepared from optochin-sensitive and optochin-resistant strains were similar.
从对奥普托欣耐药的突变肺炎球菌制备的洗涤细胞悬液和无细胞提取物的亚甲蓝（MB）还原酶活性，比从对奥普托欣敏感的亲本菌株制备的类似制剂高两倍，对奥普托欣抑制的抗性高四至八倍。对于完整细胞，盐酸奥普托欣在抑制MB还原酶活性方面的效力约为盐酸奎宁的四倍。然而，对于无细胞提取物，两种药物具有相似的抑制活性。葡萄糖摄取不受盐酸奥普托欣影响，对奥普托欣敏感和耐药的肺炎球菌具有相似的葡萄糖摄取模式。从对奥普托欣耐药的肺炎球菌制备的无细胞提取物的黄递酶活性，比从对奥普托欣敏感的亲本菌株制备的无细胞提取物高两倍，对奥普托欣抑制的抗性高四至八倍。从对奥普托欣敏感和耐药菌株制备的无细胞提取物的黄素浓度相似。
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