2,4-二甲苯酚的细菌代谢
The bacterial metabolism of 2,4-xylenol.
作者信息
Chapman P J, Hopper D J
出版信息
Biochem J. 1968 Dec;110(3):491-8. doi: 10.1042/bj1100491.
Abstract
- Measurements of the rates of oxidation of various compounds by a fluorescent Pseudomonas indicated that metabolism of 2,4-xylenol was initiated by oxidation of the methyl group para to the hydroxyl group. 2. 4-Hydroxy-3-methylbenzoic acid was isolated as the product of oxidation of 2,4-xylenol by cells inhibited with alphaalpha'-bipyridyl. 3. 4-Hydroxyisophthalic acid accumulated at low oxygen concentrations when either 2,4-xylenol or 4-hydroxy-3-methylbenzoic acid was oxidized by cells grown with 2,4-xylenol. 4. When supplemented with NADH, but not with NADPH, cell extracts oxidized 4-hydroxy-3-methylbenzoic acid readily. 2-Hydroxy-5-methylbenzoic acid was not oxidized. 5. Both 4-hydroxyisophthalic acid and p-hydroxybenzoic acid were oxidized to beta-oxoadipic acid by cell extracts supplemented with either NADH or NADPH. 4,5-Dihydroxyisophthalic acid was not oxidized. 6. From measurements of oxygen consumed and carbon dioxide evolved it was concluded that protocatechuic acid is an intermediate in the conversion of 4-hydroxyisophthalic acid into beta-oxoadipic acid.
摘要
- 对荧光假单胞菌氧化各种化合物的速率进行测量表明，2,4-二甲苯酚的代谢是由羟基对位甲基的氧化引发的。2. 4-羟基-3-甲基苯甲酸是用α,α'-联吡啶抑制的细胞氧化2,4-二甲苯酚的产物。3. 当用2,4-二甲苯酚培养的细胞氧化2,4-二甲苯酚或4-羟基-3-甲基苯甲酸时，在低氧浓度下会积累4-羟基间苯二甲酸。4. 当补充NADH而非NADPH时，细胞提取物很容易氧化4-羟基-3-甲基苯甲酸。2-羟基-5-甲基苯甲酸未被氧化。5. 补充有NADH或NADPH的细胞提取物将4-羟基间苯二甲酸和对羟基苯甲酸都氧化为β-氧代己二酸。4,5-二羟基间苯二甲酸未被氧化。6. 通过测量消耗的氧气和释放的二氧化碳得出结论，原儿茶酸是4-羟基间苯二甲酸转化为β-氧代己二酸过程中的中间产物。
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