Yamamoto Y
Plant Physiol. 1969 Feb;44(2):262-6. doi: 10.1104/pp.44.2.262.
Mitochondria from cotyledons of Vigna sesquipedalis (L.) Fruwirth (starchy seed) showed no NAD-isocitric dehydrogenase (NAD-IDH) activity by the methods which have been known to be useful for the detection of NAD-IDH in mitochondria of plants including castor bean and alaska pea. When the Vigna cotyledon mitochondria were treated with glycerol, NAD-IDH activity appeared and NADP-isocitric dehydrogenase (NADP-IDH) activity was inhibited. The inhibition of mitochondrial NADP-IDH by glycerol was overcome by the addition of excess NADP. On the other hand, NADP-IDH activity in the soluble fraction of cell components was only slightly inhibited by glycerol and no NAD-IDH activity was elicited. It was postulated that NADP-IDH in mitochondria is converted to NAD-IDH by glycerol and back to NADP-IDH with NADP by the alteration in the spatial configuration of the enzyme. However, there could be 2 proteins as the other possibility. The NADP-IDH in the soluble fraction which is not subject to such alteration is different from the mitochondrial NADP-IDH.
对于已知可用于检测包括蓖麻和阿拉斯加豌豆在内的植物线粒体中NAD - 异柠檬酸脱氢酶（NAD - IDH）的方法，长豇豆（Vigna sesquipedalis (L.) Fruwirth）（淀粉种子）子叶中的线粒体未显示出NAD - IDH活性。当长豇豆子叶线粒体用甘油处理时，NAD - IDH活性出现，而NADP - 异柠檬酸脱氢酶（NADP - IDH）活性受到抑制。添加过量的NADP可克服甘油对线粒体NADP - IDH的抑制作用。另一方面，甘油仅轻微抑制细胞组分可溶部分中的NADP - IDH活性，且未引发NAD - IDH活性。据推测，线粒体中的NADP - IDH通过甘油转化为NAD - IDH，并通过酶空间构型的改变随NADP变回NADP - IDH。然而，作为另一种可能性，可能存在两种蛋白质。不受这种改变影响的可溶部分中的NADP - IDH与线粒体NADP - IDH不同。
