Harvey A L, Robertson J G, Witkowski J A
J Neurol Sci. 1979 Mar;41(1):115-22. doi: 10.1016/0022-510x(79)90145-x.
Human foetal skeletal muscle was grown in explant cultures and the development of myotubes was monitored morphologically and by the development of membrane potential and acetycholine sensitivity. Migration of uninucleate cells from the explants occurred in the first day of culture and formation of multinucleated myotubes took place between 7 to 10 days. Early myotubes were variable in appearance, being either flat and nonrefractile or narrow and cylindrical. Some cross-striated cells were observed. Resting membrane potentials were around -25mV and there was great variation in myotube sensitivity to acetylcholine. After about 6 weeks in culture most myotubes were of the refractile type. Many had hypolemmal nuclei and were cross-striated; some contracted spontaneously. All myotubes had developed high sensitivity to acetylcholine although there had been no increase in resting membrane potential.
人胎儿骨骼肌在组织块培养中生长，并通过形态学、膜电位的变化以及乙酰胆碱敏感性来监测肌管的发育。培养第一天，单核细胞从组织块中迁移出来，多核肌管在7至10天内形成。早期肌管外观各异，有的扁平且无折射性，有的狭窄呈圆柱形。观察到一些横纹肌细胞。静息膜电位约为-25mV，肌管对乙酰胆碱的敏感性差异很大。培养约6周后，大多数肌管为折射型。许多肌管有核下核且有横纹；一些能自发收缩。尽管静息膜电位没有增加，但所有肌管对乙酰胆碱都已产生高敏感性。
