噬菌体T4感染后新的核糖核苷酸还原酶系统。
A new ribonucleotide reductase system after infection with phage T4.
作者信息
Berglund O, Karlström O, Reichard P
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):829-35. doi: 10.1073/pnas.62.3.829.
Abstract
The ribonucleotide reductase system of Escherichia coli B participates in the biosynthesis of DNA by reducing ribonucleoside diphosphates to the corresponding deoxyribonucleotides. The enzyme is regulated in a complicated way by allosteric modifiers. We now find that infection of E. coli with the bacteriophage T4 results in the appearance of a new ribonucleotide reductase system which shows a somewhat different pattern of regulation. Two new proteins, provisionally called fractions A and B, were purified from the extracts of infected bacteria. The reduction of ribonucleotides by these two fractions required the addition of either dithiothreitol or TPNH and E. coli thioredoxin reductase. Mutants of T4 which lacked fraction A activity were obtained. Fraction B may be a virus-induced thioredoxin.
摘要
大肠杆菌B的核糖核苷酸还原酶系统通过将核糖核苷二磷酸还原为相应的脱氧核糖核苷酸参与DNA的生物合成。该酶受到变构调节剂的复杂调控。我们现在发现，用噬菌体T4感染大肠杆菌会导致出现一种新的核糖核苷酸还原酶系统，其调控模式略有不同。从感染细菌的提取物中纯化出了两种新蛋白质，暂称为组分A和组分B。这两种组分还原核糖核苷酸需要添加二硫苏糖醇或TPNH以及大肠杆菌硫氧还蛋白还原酶。获得了缺乏组分A活性的T4突变体。组分B可能是病毒诱导的硫氧还蛋白。
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本文引用的文献
1
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J Am Chem Soc. 1965 May 20;87:2257-65. doi: 10.1021/ja01088a027.
2
3
THYMIDINE-REQUIRING MUTANTS OF PHAGE T4.噬菌体T4的胸腺嘧啶核苷需求型突变体
Proc Natl Acad Sci U S A. 1963 Sep;50(3):526-32. doi: 10.1073/pnas.50.3.526.
4
Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4.用噬菌体T4的一些琥珀突变体感染大肠杆菌后早期酶的合成及其调控
Proc Natl Acad Sci U S A. 1962 Feb;48(2):293-302. doi: 10.1073/pnas.48.2.293.
5
6
Resolution of phage-initiated and normal host thymidylate synthetases of Escherichia coli.大肠杆菌噬菌体引发的和正常宿主胸苷酸合成酶的解析
Proc Natl Acad Sci U S A. 1962 Feb;48(2):242-7. doi: 10.1073/pnas.48.2.242.
7
8
9
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10
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J Biol Chem. 1953 Nov;205(1):291-5.
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