猪肝细胞质中烟酰胺腺嘌呤二核苷酸磷酸依赖性异柠檬酸脱氢酶的纯化及性质
Purification and properties of the nicotinamide-adenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm.
作者信息
Illingworth J A, Tipton K F
出版信息
Biochem J. 1970 Jun;118(2):253-8. doi: 10.1042/bj1180253.
Abstract
The NADP-dependent isocitrate dehydrogenase from pig liver soluble fraction was purified over 500-fold with an overall yield of 25%. The purified enzyme, which is homogeneous by all the usual criteria, has a molecular weight of about 75000 and is composed of two identical subunits. This has been demonstrated by ultracentrifugation, fluorescence titration and peptide ;fingerprinting'. The maximal turnover number, extinction coefficients at 280nm and 260nm and amino acid analysis are described.
摘要
从猪肝可溶性部分纯化得到的依赖烟酰胺腺嘌呤二核苷酸磷酸（NADP）的异柠檬酸脱氢酶，纯化倍数超过500倍，总产率为25%。通过所有常规标准鉴定，该纯化酶均一，分子量约为75000，由两个相同的亚基组成。超速离心、荧光滴定和肽“指纹图谱”已证实了这一点。文中还描述了该酶的最大周转数、280nm和260nm处的消光系数以及氨基酸分析结果。
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GRAPHICAL DETERMINATION OF EQUILIBRIUM CONSTANTS.平衡常数的图形测定
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