核糖核酸的热扰动微分光谱。I. 水合作用影响。
Thermal perturbation differential spectra of ribonucleic acids. I. Hydration effects.
作者信息
Frechet D, Ehrlich R, Remy P
出版信息
Nucleic Acids Res. 1979 Dec 11;7(7):1965-80. doi: 10.1093/nar/7.7.1965.
A relatively important change in UV absorption is observed upon thermal perturbation of nucleotide solutions. Comparison of these thermal perturbation spectra of nucleic acid residues with solvent perturbation spectra of the same compounds suggests that this spectral change can most probably be attributed to temperature induced hydration change of the bases. This conclusion is confirmed by the results obtained from acid-base perturbation spectra of these nucleotides as well as thermal perturbation spectra of nucleotides containing modified bases. It is shown that this temperature dependent change in UV absorption is also present in dinucleoside monophosphates. In that case, this effect is superimposed upon the well known change in absorbance due to the unstacking of the bases during heating.
在对核苷酸溶液进行热扰动时，观察到紫外吸收发生了相对重要的变化。将这些核酸残基的热扰动光谱与相同化合物的溶剂扰动光谱进行比较表明，这种光谱变化很可能归因于碱基的温度诱导水合变化。这些核苷酸的酸碱扰动光谱以及含修饰碱基的核苷酸的热扰动光谱所得到的结果证实了这一结论。结果表明，这种紫外吸收的温度依赖性变化在单磷酸二核苷中也存在。在这种情况下，这种效应叠加在加热过程中由于碱基解堆叠而导致的众所周知的吸光度变化之上。
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