肾形海鳃生物发光过程中氧化二氧化碳产生的机制。
Mechanism of oxidative carbon dioxide production during Renilla reniformis bioluminescence.
作者信息
DeLuca M, Dempsey M E, Hori K, Wampler J E, Cormier M J
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1658-60. doi: 10.1073/pnas.68.7.1658.
Abstract
The oxidation of luciferin catalyzed by sea pansy luciferase results in the emission of light. Molecular oxygen is required and carbon dioxide is produced. When the reaction occurs in the presence of H(2) (18)O, both of the oxygens of the carbon dioxide are labeled. One of the oxygens arises from the nonenzymic exchange of the ketone group of the substrate; the other oxygen is incorporated during the enzymic oxidation of the luciferin. When the reaction is carried out in the presence of (18)O(2), neither of the oxygens of the carbon dioxide is labeled. Thus the source of oxygen in the carbon dioxide is water. A mechanism for the oxidative reaction is proposed.
摘要
海仙人掌荧光素酶催化荧光素的氧化反应会导致发光。该反应需要分子氧并产生二氧化碳。当反应在H₂¹⁸O存在的情况下发生时，二氧化碳中的两个氧原子都被标记。其中一个氧原子来自底物酮基的非酶促交换；另一个氧原子是在荧光素的酶促氧化过程中掺入的。当反应在¹⁸O₂存在的情况下进行时，二氧化碳中的氧原子都不会被标记。因此，二氧化碳中氧的来源是水。本文提出了氧化反应的机制。
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