[Complementary interaction of multialkylated polyribonucleotides].

Effect of modification of polyinosinic acid (poly(I)) by alkylating agent 4-N,N-bis (beta-chloroethyl) aminobenzaldedehyde on its complementary interaction with polycytidylic acid (poly(C)) was investigated. The data demonstrate that when no more than 10% of inosine residues are modified poly(I) still retains its capacity to form complementary complex with poly (C) this capacity disappears after modification of 11--13% of inosine residues. However when more than 3,5% of inosine residues were modified the poly(I). .poly(C) complex became sensitive to RNAse T1 and its Tm decreases, indicating that single-stranded regions (loops) are formed under these conditions. The data suggest that modified polynucleotides carrying alkylating groups on their surface can be applied for the directed action on the complementary regions of the genome.