Hemmings W A, Jones R E
Immunology. 1974 Sep;27(3):343-50.
Twelve-day-old rats were fed a mixture of fast and slow chromatographically prepared fractions of bovine IgG, labelled with I and I. The pooled young rat sera were submitted to analysis by electrofocusing. The results show a wide variation of concentration quotients between the different pI peaks of IgG, with the more basic fractions being transmitted more readily in both fractions. One slow IgG peak (pI 7.8) in particular is transmitted very readily. These findings reinforce the concept of selectivity in transmission. The possible use of the young rat gut as a test for protein integrity is discussed.
给12日龄的大鼠喂食经快速和慢速色谱法制备的、用I和I标记的牛IgG组分混合物。将合并的幼鼠血清进行电聚焦分析。结果显示，IgG不同pI峰之间的浓度商差异很大，在两个组分中，碱性更强的组分更容易被传递。特别是一个慢速IgG峰（pI 7.8）很容易被传递。这些发现强化了传递中的选择性概念。讨论了将幼鼠肠道用作蛋白质完整性测试的可能性。
