Fucci L, Gaudio L, Rao R, Spanò A, Carfagna M
Biochem Genet. 1979 Oct;17(9-10):825-36. doi: 10.1007/BF00504306.
Phosphoglucomutase (PGM) of adult stage in Drosophilia melanogaster has been characterized by gel filtration, ion-exchange chromatography, and isoelectric focusing. The two common electrophoretic variants, PGMA and PGMB, differ with respect to their kinetic and stability parameters. PGMA is more thermostable than PGMB but shows the same pH optimum, equal dependence on Mg2+, and identical molecular weight. There is no significant kinetic difference between the two allozymes at the optimum pH values, but at pH 6.0 the Km value for glucose-1,6-diphosphate of PGMB is significantly higher than that of PGMA. This difference might explain the observed selective advantage of the PgmA allele in population studies.
黑腹果蝇成虫阶段的磷酸葡萄糖变位酶（PGM）已通过凝胶过滤、离子交换色谱和等电聚焦进行了表征。两种常见的电泳变体PGMA和PGMB在动力学和稳定性参数方面存在差异。PGMA比PGMB更耐热，但具有相同的最适pH值，对Mg2+的依赖性相同，分子量也相同。在最适pH值下，这两种同工酶之间没有显著的动力学差异，但在pH 6.0时，PGMB的1,6-二磷酸葡萄糖的Km值明显高于PGMA。这种差异可能解释了在群体研究中观察到的PgmA等位基因的选择优势。
