衣藻中叶绿体核糖体的生物发生。核糖体形成受阻突变体的筛选与鉴定。
Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation.
作者信息
Harris E H, Boynton J E, Gillham N W
出版信息
J Cell Biol. 1974 Oct;63(1):160-79. doi: 10.1083/jcb.63.1.160.
Abstract
Chloroplast protein synthesis in Chlamydomonas reinhardtii is dispensable when cells are provided acetate as a carbon source. Mutants defective in synthesis, assembly, or function of chloroplast ribosomes are therefore conditionally viable. Positive selection of nonphotosynthetic cells on arsenate has been combined with a simple screening procedure to isolate mutants with a broad spectrum of defects in chloroplast protein synthesis. Eight new mutants deficient in chloroplast ribosomes have been isolated. Three of these have been characterized genetically and phenotypically, and compared with two previously described ribosome mutants, ac-20 and cr-1. A working model of ribosome assembly is proposed which suggests possible biochemical roles for these five Mendelian gene loci.
摘要
当莱茵衣藻细胞以乙酸盐作为碳源时，叶绿体蛋白质合成是可有可无的。因此，叶绿体核糖体合成、组装或功能存在缺陷的突变体是条件存活的。利用砷酸盐对非光合细胞进行正向选择，并结合一个简单的筛选程序，以分离出叶绿体蛋白质合成存在广泛缺陷的突变体。已经分离出八个新的叶绿体核糖体缺陷突变体。其中三个已在遗传和表型上进行了表征，并与之前描述的两个核糖体突变体ac-20和cr-1进行了比较。提出了一个核糖体组装的工作模型，该模型暗示了这五个孟德尔基因座可能的生化作用。
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