用于固定生物活性化合物和亲和层析的稳定、高容量且不带电荷的琼脂糖衍生物。 - Suppr

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超能文献

Stable, high capacity and non charged agarose derivatives for immobilization of biologically active compounds and for affinity chromatography.

作者信息

Wilchek M, Miron T

出版信息

Mol Cell Biochem. 1974 Oct 30;4(3):181-7. doi: 10.1007/BF01731479.

DOI:10.1007/BF01731479

PMID:4427591

Abstract

摘要

相似文献

Stable, high capacity and non charged agarose derivatives for immobilization of biologically active compounds and for affinity chromatography.用于固定生物活性化合物和亲和层析的稳定、高容量且不带电荷的琼脂糖衍生物。

Mol Cell Biochem. 1974 Oct 30;4(3):181-7. doi: 10.1007/BF01731479.

Recent developments in protein chromatography involving hydrophobic interactions.蛋白质色谱法中涉及疏水相互作用的最新进展。

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Acyl azide derivatives in affinity chromatography. Immobilization of enzymatically active trypsin on beaded agarose and porous glass.

Biochim Biophys Acta. 1973 Jul 12;317(1):20-7.

Organomercurial agarose.

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Covalent immobilization of proteins to N-hydroxysuccinimide ester derivatives of agarose. Effect of protein charge on immobilization.

Biochim Biophys Acta. 1981 Sep 29;670(2):163-9. doi: 10.1016/0005-2795(81)90004-0.

Polymers coupled to agarose as stable and high capacity spacers.

Methods Enzymol. 1974;34:72-6. doi: 10.1016/s0076-6879(74)34008-6.

The derivatization of oxidized polysaccharides for protein immobilization and affinity chromatography.用于蛋白质固定化和亲和色谱的氧化多糖衍生化

Biochim Biophys Acta. 1976 Mar 25;428(1):157-65. doi: 10.1016/0304-4165(76)90117-3.

Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifuctional oxiranes.生物特异性亲和色谱吸附剂的制备。通过双功能环氧乙烷将含基团的配体连接到不溶性聚合物上。

J Chromatogr. 1974 Mar 13;90(1):87-98. doi: 10.1016/s0021-9673(01)94777-6.

Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.DNA与琼脂糖的共价连接。改进的合成方法及其在亲和色谱中的应用。

Eur J Biochem. 1975 Jun;54(2):411-8. doi: 10.1111/j.1432-1033.1975.tb04151.x.

Isolation of vitamin B12-binding proteins using affinity chromatography. I. Preparation and properties of vitamin B12-sepharose.

J Biol Chem. 1972 Dec 10;247(23):7695-701.

引用本文的文献

Biochemical evidence for a separate, MHC-linked locus encoding H-2.28 antigens.关于一个独立的、与主要组织相容性复合体（MHC）连锁的基因座编码H-2.28抗原的生化证据。

Immunogenetics. 1980;10(1):67-82. doi: 10.1007/BF01561553.

Evidence for the presence of di- and triphospho pyridine nucleotide dehydrogenase derivatives as consistent contaminants of purified beef heart cytochrome-c oxidase.

J Bioenerg Biomembr. 1976 Dec;8(6):271-92. doi: 10.1007/BF00765157.

本文引用的文献

Chemical coupling of proteins to agarose.蛋白质与琼脂糖的化学偶联。

Nature. 1967 Sep 30;215(5109):1491-2. doi: 10.1038/2151491a0.

Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose.使用溴乙酰纤维素制备的免疫吸附剂纯化抗体。

Immunochemistry. 1967 Jan;4(1):11-22. doi: 10.1016/0019-2791(67)90192-9.

Chemical fixation of chymotrypsin to water-insoluble crosslinked dextran (sephadex) and solubilization of the enzyme derivatives by means of dextranase.

Biopolymers. 1970;9(4):401-13. doi: 10.1002/bip.1970.360090403.

Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。

J Biol Chem. 1970 Jun;245(12):3059-65.

The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.交联聚丙烯酰胺微球的衍生化。为制备特殊用途的生化吸附剂而对官能团进行的可控引入。

Biochemistry. 1969 Oct;8(10):4074-82. doi: 10.1021/bi00838a026.

Selective enzyme purification by affinity chromatography.通过亲和色谱法进行选择性酶纯化。

Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. doi: 10.1073/pnas.61.2.636.

Immobilization of enzymes through non-covalent binding to substituted agaroses.通过与取代琼脂糖非共价结合来固定化酶。

Biochem Biophys Res Commun. 1973 Aug 21;53(4):1137-44. doi: 10.1016/0006-291x(73)90583-4.

Use of isoelectric focusing to characterize the bonds established during coupling of CNBr-activated amylodextrin to subtilisin type novo.使用等电聚焦来表征在溴化氰活化的支链糊精与新型枯草杆菌蛋白酶偶联过程中形成的键。

FEBS Lett. 1973 Jan 15;29(2):167-9. doi: 10.1016/0014-5793(73)80552-6.

Protein binding by agarose carrying hydrophobic groups in conjunction with charges.通过带有疏水基团并结合电荷的琼脂糖进行蛋白质结合。

Biochem Biophys Res Commun. 1973 Feb 5;50(3):751-7. doi: 10.1016/0006-291x(73)91308-9.

Hydrocarbon-coated sepharoses. Use in the purification of glycogen phosphorylase.碳氢化合物包被的琼脂糖凝胶。用于糖原磷酸化酶的纯化。

Biochem Biophys Res Commun. 1972 Oct 17;49(2):383-90. doi: 10.1016/0006-291x(72)90422-6.

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