采用高效液相色谱法测定生物流体中抗变应原剂2-甲氧基-11-氧代-11H-吡啶并[2,1-b]喹唑啉-8-羧酸的含量。

Determination of the anti-allergenic agent, 2-methoxy-11-oxo-11H-pyrido[2,1-b]quinazoline-8-carboxylic acid, in biological fluids by high-performance liquid chromatography.

作者信息

Strojny N, de Silva J A

出版信息

J Chromatogr. 1979 Nov 21;179(2):311-21. doi: 10.1016/s0021-9673(00)83833-9.

Abstract

A rapid, sensitive, and specific high performance liquid chromatographic (HPLC) assay was developed for the determination of 2-methoxy-11-oxo-11H-pyrido-[2,1-b]quinazoline-8-carboxylic acid (I) from biological fluids. The overall recovery from blood and plasma is 69 +/- 10% (S.D.) and 84 +/- 6% (S.D.), respectively, and the sensitivity limit of quantitation is 100 ng/ml by UV absorption and 5 ng/ml by fluorescence detection using a 1 ml specimen. The assay was used in the determination of blood levels of compound in the Rhesus monkey following intravenous administration of a 10 mg/kg dose, and of blood and urine levels of compound I in a dog following intravenous and oral administration of a 1 mg/kg dose.

摘要

已开发出一种快速、灵敏且特异的高效液相色谱（HPLC）分析法，用于测定生物流体中的2-甲氧基-11-氧代-11H-吡啶并-[2,1-b]喹唑啉-8-羧酸（I）。血液和血浆的总回收率分别为69±10%（标准差）和84±6%（标准差），使用1ml样本时，紫外吸收法定量的灵敏度极限为100ng/ml，荧光检测法定量的灵敏度极限为5ng/ml。该分析法用于在恒河猴静脉注射10mg/kg剂量后测定化合物的血药浓度，以及在犬静脉注射和口服1mg/kg剂量后测定化合物I的血药浓度和尿药浓度。