黄嘌呤在离子交换树脂上的色谱分析

Chromatography of xanthines on ion-exchange resins.

作者信息

Walton H F, Eiceman G A, Otto J L

出版信息

J Chromatogr. 1979 Nov 28;180(1):145-56. doi: 10.1016/s0021-9673(00)80184-3.

Abstract

Short columns of a 4% crosslinked cation-exchange resin gave good chromatography of xanthines, including caffeine, theophylline and hypoxanthine, and related polar aromatic compounds. Elution volumes and sequences can be modified by changing pH, solvent composition and resin counter-ion. A macroporous cation-exchange resin showed exaggerated counter-ion effects. A method is described for determining caffeine and theophylline in blood serum, using the 4% crosslinked resin with aqueous sodium phosphate eluent of pH 7.5; the temperature was 65 degrees. Detection limits are 10 ng and less.

摘要

4%交联阳离子交换树脂的短柱能对黄嘌呤类物质（包括咖啡因、茶碱和次黄嘌呤）以及相关极性芳香族化合物进行良好的色谱分离。通过改变pH值、溶剂组成和树脂抗衡离子，可以改变洗脱体积和顺序。一种大孔阳离子交换树脂显示出夸张的抗衡离子效应。本文描述了一种使用4%交联树脂和pH值为7.5的磷酸钠水溶液洗脱剂测定血清中咖啡因和茶碱的方法；温度为65摄氏度。检测限为10纳克及更低。