肥胖高血糖（ob/ob）小鼠的肝脏单胺氧化酶
Liver monoamine oxidase in the obese-hyperglycaemic (ob/ob) mouse.
作者信息
Tong J H, Limson-Zamora M, D'Iorio A, Bégin-Heick N
出版信息
Biochem J. 1979 Mar 1;177(3):943-9. doi: 10.1042/bj1770943.
Abstract
- The specific activity of monoamine oxidase was found to be greater in liver mitochondria from ob/ob mice than from lean mice. The activities of marker enzymes were similar in both tissues. 2. Experiments with various substrates (5-hydroxytryptamine, benzylamine and tyramine) and inhibitors (clorgyline and deprenyl) indicated that, unlike rat liver mitochondria, mouse liver mitochondria contain a predominance of the B-form of monoamine oxidase. 3. The Km values for lean and ob/ob mice were the same for any given substrate and were in the increasing order 5-hydroxytryptamine less than tyramine less than benzylamine. Vmax. was approximately 50% greater in obese than in lean mice. 4. Extraction of liver mitochondria with acetone/water or acetone/water/NH3 to remove lipids decreased the enzyme activity relatively more in obese- than in lean-mice preparations, but residual activity was the same in both preparations.
摘要
- 研究发现，ob/ob小鼠肝脏线粒体中，单胺氧化酶的比活性高于瘦小鼠。两种组织中标记酶的活性相似。2. 用各种底物（5-羟色胺、苄胺和酪胺）和抑制剂（氯吉兰和司来吉兰）进行的实验表明，与大鼠肝脏线粒体不同，小鼠肝脏线粒体中主要含有B型单胺氧化酶。3. 对于任何给定底物，瘦小鼠和ob/ob小鼠的Km值相同，且呈5-羟色胺＜酪胺＜苄胺的递增顺序。肥胖小鼠的Vmax比瘦小鼠大约高50%。4. 用丙酮/水或丙酮/水/氨提取肝脏线粒体以去除脂质，肥胖小鼠制剂中的酶活性相对瘦小鼠制剂下降得更多，但两种制剂中的残余活性相同。
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