给予二甲基亚硝胺后叙利亚金仓鼠肝脏核糖核酸中甲基化碱基的持久性。
Persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine.
作者信息
Margison G P, Margison J M, Montesano R
出版信息
Biochem J. 1979 Mar 1;177(3):967-73. doi: 10.1042/bj1770967.
Abstract
- Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. The chemical alkyation products, 7-methylguanine, 3-methylcytosine, O6-methylguanosine and 1-methyladenosine, were measured after acidic or enzymic hydrolysis of the RNA to bases or mononucleosides followed by ion-exchange chromatography. 3. Between 7 and 96 h, the relative amounts of alkylation products did not change with time even though the absolute amounts fell by approx. 80% and 51% after the high and low doses respectively. 4. The results suggest that base specific excision repair does not exist for RNA alkylation products in this experimental system.
摘要
- 在以25毫克/千克或2.5毫克/千克体重给予[14C]二甲基亚硝胺后，长达96小时内分离叙利亚金黄地鼠肝脏核糖体RNA。2. 在RNA经酸性或酶水解为碱基或单核苷后，通过离子交换色谱法测量化学烷基化产物7-甲基鸟嘌呤、3-甲基胞嘧啶、O6-甲基鸟苷和1-甲基腺苷。3. 在7至96小时之间，烷基化产物的相对量不随时间变化，尽管高剂量和低剂量后绝对量分别下降了约80%和51%。4. 结果表明，在该实验系统中，RNA烷基化产物不存在碱基特异性切除修复。
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