一种利用芘激基缔合物形成来测量膜微粘度的方法。应用于人类红细胞血影。
A method for measuring membrane microviscosity using pyrene excimer formation. Application to human erythrocyte ghosts.
作者信息
Dembo M, Glushko V, Aberlin M E, Sonenberg M
出版信息
Biochim Biophys Acta. 1979 Apr 4;552(2):201-11. doi: 10.1016/0005-2736(79)90277-3.
In order to determine the microviscosity of human erythrocyte membrane suspensions, a method has been developed which is based on pyrene excimer formation. First, measurements of partitioning of pyrene into membranes, in conjunction with known values for the volume of the lipid compartment of erythrocyte ghosts are used to determine the concentration of pyrene in the membrane lipid. Secondly, reported measurements of the diffusion constants of aromatic hydrocarbons similar in structure to pyrene, are used to derive an empirical equation relating solvent viscosity and the diffusion constant of pyrene. Then, measurements of pyrene excimer formation in a series of solvents ranging up to several poise in viscosity are used to determine that the interaction diameter of the excimer formation reaction is 3 +/- 1 A. Finally all these data are brought together in order to conclude that the viscosity of the lipid in the human erythrocyte ghost is 8.0, 4.0 and 1.6 P at 10, 25 and 40 degrees C, respectively.
为了测定人红细胞膜悬浮液的微粘度，已开发出一种基于芘激基缔合物形成的方法。首先，通过测量芘在膜中的分配，并结合已知的红细胞影脂质区室体积值，来确定膜脂质中芘的浓度。其次，利用已报道的与芘结构相似的芳烃扩散常数测量值，推导出一个将溶剂粘度与芘扩散常数相关联的经验方程。然后，在一系列粘度高达几泊的溶剂中测量芘激基缔合物的形成，以确定激基缔合物形成反应的相互作用直径为3±1埃。最后，综合所有这些数据得出结论，人红细胞影中脂质的粘度在10℃、25℃和40℃时分别为8.0、4.0和1.6泊。
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