牛角瓜乳汁中蛋白酶的研究。I. 两种含糖蛋白酶的纯化及某些性质
Studies on proteinases from Calotropis gigantea latex. I. Purification and some properties of two proteinases containing carbohydrate.
作者信息
Abraham K I, Joshi P N
出版信息
Biochim Biophys Acta. 1979 May 10;568(1):111-9. doi: 10.1016/0005-2744(79)90278-x.
PMID:444537
Abstract
Two proteinase containing carbohydrate, called calotropain-FI and calotropain-FII, were purified from Calotropis gigantea latex by CM-Sephadex C-50 chromatography. Both calotropain-FI and FII were found to be homogeneous by rechromatography on CM-Sephadex C-50, gel filtration on Sephadex G-100, electrophoresis on polyacrylamide gel and by N-terminal amino acid analysis. Some properties of these enzymes are reported.
摘要
通过CM-葡聚糖凝胶C-50色谱法从牛角瓜乳汁中纯化出两种含碳水化合物的蛋白酶，分别称为牛角瓜蛋白酶-FI和牛角瓜蛋白酶-FII。通过在CM-葡聚糖凝胶C-50上再次色谱分离、在葡聚糖凝胶G-100上进行凝胶过滤、在聚丙烯酰胺凝胶上进行电泳以及通过N端氨基酸分析，发现牛角瓜蛋白酶-FI和FII均为均一的。报道了这些酶的一些性质。
Zotero 插件