Andersen K
Biochim Biophys Acta. 1979 Jun 1;585(1):1-11. doi: 10.1016/0304-4165(79)90319-2.
Use of a bacterial system has allowed the isolation of several mutationally altered species of ribulose-1,5-bisphosphate carboxylase/oxygenase having a wide range of catalytic activities. Biochemical and serological techniques were used to classify the mutant strains into three groups: (1) no detectable ribulose-1,5-bisphosphate carboxylase/oxygenase protein present (both subunits missing); (2) mutant strains having ribulose-1,5-bisphosphate carboxylase/oxygenases with lowered specific activities; and (3) mutant strains producing large quantities of catalytically inactive ribulose-1,5-bisphosphate carboxylase/oxygenase.
利用细菌系统已分离出几种具有广泛催化活性的经突变改变的1,5-二磷酸核酮糖羧化酶/加氧酶物种。采用生化和血清学技术将突变菌株分为三组：(1) 不存在可检测到的1,5-二磷酸核酮糖羧化酶/加氧酶蛋白（两个亚基均缺失）；(2) 具有比活性降低的1,5-二磷酸核酮糖羧化酶/加氧酶的突变菌株；(3) 产生大量无催化活性的1,5-二磷酸核酮糖羧化酶/加氧酶的突变菌株。
