葡糖二酸分解代谢的研究：食酸假单胞菌葡糖二酸水解酶的氧化脱氧葡糖二酸醛缩酶活性
Studies on glucarate catabolism: the oxodeoxyglucarate aldolase activity of glucarate hydro-lyase from Pseudomonas acidovorans.
作者信息
Jeffcoat R
出版信息
Biochem J. 1974 May;139(2):477-80. doi: 10.1042/bj1390477.
Abstract
Glucarate hydro-lyase was isolated and purified to near homogeneity from cells of Pseudomonas acidovorans grown on glucarate. By using gel filtration and ion-exchange chromatography, it was shown that the oxodeoxyglucarate aldolase activity observed in such extracts is associated with the glucarate hydro-lyase protein.
摘要
从以葡糖二酸为碳源生长的食酸假单胞菌细胞中分离并纯化葡糖二酸水解酶，使其接近均一。通过凝胶过滤和离子交换色谱法表明，在此类提取物中观察到的氧化脱氧葡糖二酸醛缩酶活性与葡糖二酸水解酶蛋白相关。
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