单剂量口服给药后血清中低水平氨苄西林的快速、精确比浊法测定。
Rapid, precise, turbidometric assay for low levels of ampicillin in serum after single-dose oral administration.
作者信息
Whyatt P L, Dann R E, Slywka G W, Meyer M C
出版信息
Antimicrob Agents Chemother. 1974 Dec;6(6):811-4. doi: 10.1128/AAC.6.6.811.
A turbidometric assay is described for the quantitative measurement of ampicillin in serum. Standard curves prepared with known concentrations of ampicillin in serum exhibited acceptable linearity over a concentration range of approximately 0.2 to 1.8 mug/ml. Data are presented to show the excellent precision of the assay and the application of the assay to clinical studies. The advantages of this method over other procedures are discussed. Because of the questionable stability of ampicillin, samples containing known concentrations of ampicillin in serum were assayed after storage for various lengths of time. Serum samples maintained in the frozen state until the time of assay exhibited approximately 12% degradation after 7 days, whereas those samples which were subjected to repeated thawing and refreezing exhibited approximately 25% degradation after the same time interval.
本文描述了一种用于定量测定血清中氨苄西林的比浊法。用已知浓度的血清氨苄西林制备的标准曲线在约0.2至1.8微克/毫升的浓度范围内表现出可接受的线性。文中给出的数据表明了该测定方法具有出色的精密度以及该方法在临床研究中的应用。讨论了该方法相对于其他方法的优点。由于氨苄西林的稳定性存在疑问，对含有已知浓度氨苄西林的血清样本在储存不同时间后进行了测定。在冷冻状态下保存至测定时的血清样本在7天后显示出约12%的降解，而那些经历反复冻融的样本在相同时间间隔后显示出约25%的降解。
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