在从幼鼠和成年大鼠大脑中获取的主要细胞核类别中两种脱氧核糖核酸聚合酶活性的出现情况。
The occurrence of two types of deoxyribonucleic acid polymerase activity in the main classes of nuclei obtained from the brains of infant and adult rats.
作者信息
Stambolova M A, Cox D, Mathias A P
出版信息
Biochem J. 1974 Apr;140(1):65-71. doi: 10.1042/bj1400065.
Abstract
- The influence of exogenous or activated DNA template on the DNA polymerase activity in the different types of intact nuclei from rat brain tissue was determined. The different amounts or physical state of the DNA template did not produce significant differences in the relative distribution of the DNA polymerase activity between the separate groups of nuclei. 2. The DNA polymerase activities, fractionated by sucrose gradient centrifugation into enzyme A and enzyme B, were found to be present in the extracts of all types of rat brain nuclei. The distribution of these two activities in the ;particulate' and ;soluble' fractions of the separate groups of nuclei from 10-day-old and adult rats was studied. The findings are related to the DNA-synthetic activity in vivo of the intact nuclei and the possible biological functions of the DNA polymerase activities are discussed.
摘要
- 测定了外源性或活化的DNA模板对大鼠脑组织不同类型完整细胞核中DNA聚合酶活性的影响。DNA模板的不同量或物理状态在各细胞核组之间DNA聚合酶活性的相对分布上未产生显著差异。2. 通过蔗糖梯度离心分离为酶A和酶B的DNA聚合酶活性，在所有类型的大鼠脑细胞核提取物中均有发现。研究了这两种活性在10日龄和成年大鼠各细胞核组的“颗粒”和“可溶性”组分中的分布。这些发现与完整细胞核在体内的DNA合成活性相关，并讨论了DNA聚合酶活性可能的生物学功能。
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The occurrence of two types of deoxyribonucleic acid polymerase activity in the main classes of nuclei obtained from the brains of infant and adult rats.在从幼鼠和成年大鼠大脑中获取的主要细胞核类别中两种脱氧核糖核酸聚合酶活性的出现情况。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ESTIMATION OF DEOXYRIBONUCLEIC ACID IN THE PRESENCE OF SIALIC ACID: APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS.在唾液酸存在的情况下对脱氧核糖核酸的估计：在人胃冲洗液分析中的应用。
Biochem J. 1965 Jun;95(3):612-20. doi: 10.1042/bj0950612.
3
4
Subcellular distribution of DNA polymerase activity in newborn rat brain and liver.新生大鼠脑和肝脏中DNA聚合酶活性的亚细胞分布。
Can J Biochem. 1971 Dec;49(12):1285-91. doi: 10.1139/o71-186.
5
DNA polymerases of newborn rat brain and liver.
Can J Biochem. 1971 Aug;49(8):978-86. doi: 10.1139/o71-142.
6
Low molecular weight deoxyribonucleic acid polymerase in mammalian cells.哺乳动物细胞中的低分子量脱氧核糖核酸聚合酶。
J Biol Chem. 1971 Sep 25;246(18):5835-7.
7
Pattern of developmental changes in two DNA polymerases of rat brain.大鼠大脑中两种DNA聚合酶的发育变化模式。
Biochim Biophys Acta. 1972 May 29;269(3):364-9. doi: 10.1016/0005-2787(72)90123-2.
8
Solubilization and characterization of a particulate form of DNA polymerase from adult rat brain nuclei.
Biochim Biophys Acta. 1972 Mar 24;262(3):397-400. doi: 10.1016/0005-2787(72)90278-x.
9
Separation and properties of DNA polymerases from developing rat brain.发育中大鼠脑内DNA聚合酶的分离与特性
Biochim Biophys Acta. 1971 Aug 12;246(1):44-50. doi: 10.1016/0005-2787(71)90070-0.
10
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