Young P G, Hunter N P
Can J Biochem. 1979 Apr;57(4):314-20. doi: 10.1139/o79-040.
The products of mitochondrial protein synthesis have been investigated in Tetrahymena after labelling with [35S]methionine in the presence of cycloheximide. The labelled proteins were analyzed by sodium dodecyl sulfate slab polyacrylamide gel electrophoresis. We have identified 13 electrophoretically discrete bands as well as 4 other bands with a more variable occurrence. These proteins ranged in apparent molecular weight from 8100 to 57,500. The cycloheximide-resistant incorporation could be blocked with chloramphenicol. The mitochondrial proteins appeared to be in a disaggregated state and were stable to agents such as trichloroacetic acid (hot or cold) and chloroform-methanol. The pattern of proteins was similar following labelling times ranging from 30 min to 3 h.
在用[35S]甲硫氨酸在放线菌酮存在下对嗜热四膜虫进行标记后，对线粒体蛋白质合成产物进行了研究。用十二烷基硫酸钠平板聚丙烯酰胺凝胶电泳分析标记的蛋白质。我们鉴定出13条电泳分离的条带以及另外4条出现情况更具变异性的条带。这些蛋白质的表观分子量在8100至57500之间。氯霉素可阻断放线菌酮抗性掺入。线粒体蛋白质似乎处于解聚状态，并且对诸如三氯乙酸（热或冷）和氯仿 - 甲醇等试剂稳定。标记时间从30分钟到3小时，蛋白质模式相似。
