Frondoza C G, Trivedi S M, Humphrey R L
Cancer Res. 1979 Jul;39(7 Pt 1):2497-500.
The early phase of LPC-1 plasmacytoma development was studied by in vivo labeling with [6-14C]arginine using its M component (immunoglobulin G 2a,kappa) as marker. At a time when M component was not detected or faint by protein staining of electrophoretograms, significant labeling of M component was detectable by autoradiography. Labeling of the M component was fairly constant for the first 10 hr but was markedly decreased from Days 1 to 7. Nadir (0 to 3% of initial 30-min value) was observed on Day 3. Recovery of M component labeling to the 30-min level was complete by Day 13. This period of marked reduction or "eclipse" in newly synthesized M component was shortened by 2 days when mice were pretreated with pristane or cyclophosphamide prior to tumor cell transfer. The eclipse period was also 2 days shorter in athymic BALB/c-nu/nu mice than in normal BALB/c mice. The eclipse period corresponds to the classical "lag" following tumor cell transfer before tumor growth can be detected by conventional methods. The sensitivity of the [6-14C]arginine pulse permits the in vivo detection of small numbers of tumor cells (as few as 10(6) cells) over the early time periods after cell transfer. Modification of eclipse by manipulating host and/or tumor cells may elucidate the accompanying cellular and biochemical events.
利用[6-14C]精氨酸进行体内标记，并以其M成分（免疫球蛋白G 2a，κ）作为标志物，对LPC-1浆细胞瘤发展的早期阶段进行了研究。在通过电泳图谱的蛋白质染色未检测到M成分或其很微弱的时候，通过放射自显影可检测到M成分有明显的标记。在最初的10小时内，M成分的标记相当稳定，但从第1天到第7天明显减少。在第3天观察到最低点（为最初30分钟值的0至3%）。到第13天，M成分标记恢复到30分钟水平。当在肿瘤细胞转移前用 pristane 或环磷酰胺对小鼠进行预处理时，新合成的M成分显著减少或“消失”的这段时间缩短了2天。无胸腺的BALB/c-nu/nu小鼠的消失期也比正常BALB/c小鼠短2天。消失期对应于肿瘤细胞转移后在通过传统方法检测到肿瘤生长之前的经典“延迟期”。[6-14C]精氨酸脉冲的敏感性允许在细胞转移后的早期时间段内体内检测少量肿瘤细胞（低至10(6)个细胞）。通过操纵宿主和/或肿瘤细胞来改变消失期可能会阐明伴随的细胞和生化事件。
