光亲和标记作为“映射”试剂的效用。利用结构相关的光亲和试剂对特定兔抗体亚群的研究。
The utility of photo-affinity labels as "mapping" reagents. A study of sub-populations of a specific rabbit antibody by using structurally related photo-affinity reagents.
作者信息
Smith R A, Knowles J R
出版信息
Biochem J. 1974 Jul;141(1):51-5. doi: 10.1042/bj1410051.
A specific rabbit antibody against the 4-azido-2-nitrophenyl determinant was photo-labelled by the homologous hapten epsilon-(4-azido-2-nitrophenyl)-l-lysine, and by the close structural isomer epsilon-(5-azido-2-nitrophenyl)-l-lysine. The extents of covalent labelling of the antibody-binding site were assessed by using radioactive haptens and exhaustive displacement dialysis, which leaves the unlabelled sites empty but largely intact. A single photolysis of hapten-antibody complex suffices to label those sites that are capable of being labelled. Although there is considerable overlap among sub-populations of antibody that will bind the two haptens non-covalently, sites that can be covalently labelled by one reagent cannot be labelled by the other.
一种针对4-叠氮基-2-硝基苯基决定簇的特异性兔抗体，被同源半抗原ε-(4-叠氮基-2-硝基苯基)-L-赖氨酸以及结构相近的异构体ε-(5-叠氮基-2-硝基苯基)-L-赖氨酸进行了光标记。通过使用放射性半抗原和彻底的置换透析来评估抗体结合位点的共价标记程度，置换透析会使未标记的位点空置但基本保持完整。半抗原-抗体复合物的单次光解就足以标记那些能够被标记的位点。尽管在非共价结合这两种半抗原的抗体亚群之间存在相当大的重叠，但能被一种试剂共价标记的位点不能被另一种试剂标记。
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