特定半抗原-抗体相互作用所特有的外在棉效应。
Extrinsic Cotton effects characteristic of specific hapten-antibody interactions.
作者信息
Glaser M, Singer S J
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2477-9. doi: 10.1073/pnas.68.10.2477.
Abstract
The reversible binding of the haptens 2,4-dinitrophenyllysine (DNP-lysine) and 2,4,6-trinitrophenyllysine (TNP-lysine) to either the nitrophenyl-binding myeloma protein MOPC-315 or to pooled mouse anti-DNP or anti-TNP antibodies produces large and characteristic extrinsic Cotton effects (in the circular dichroic spectra). Despite the similarities in binding characteristics of the three proteins, the circular dichroic spectra produced by the haptens bound to the active sites of these proteins were markedly different. Extrinsic Cotton effects, therefore, provide a powerful new probe of the structure of the reversible complex formed between a hapten and an antibody active site.
摘要
半抗原2,4-二硝基苯赖氨酸（DNP-赖氨酸）和2,4,6-三硝基苯赖氨酸（TNP-赖氨酸）与硝基苯结合性骨髓瘤蛋白MOPC-315或与混合的小鼠抗DNP或抗TNP抗体的可逆结合，会产生大的且具有特征性的外在科顿效应（在圆二色光谱中）。尽管这三种蛋白质在结合特性上有相似之处，但半抗原与这些蛋白质活性位点结合所产生的圆二色光谱却明显不同。因此，外在科顿效应为研究半抗原与抗体活性位点之间形成的可逆复合物的结构提供了一种强大的新探针。
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Biochemistry. 1964 Jul;3:996-1008. doi: 10.1021/bi00895a027.
2
Extrinsic Cotton effect and immunological properties of the p-azobenzenearsonate hapten attached to a helical amino acid copolymer.连接于螺旋氨基酸共聚物上的对氨基苯砷酸半抗原的外在柯顿效应和免疫学性质。
Biochemistry. 1970 Dec 8;9(25):4870-5. doi: 10.1021/bi00827a007.
3
The affinity-labeled residues in antibody active sites. II. Nearest-neighbor analyses.抗体活性位点中的亲和标记残基。II. 邻位分析。
Biochemistry. 1969 Nov;8(11):4523-34. doi: 10.1021/bi00839a045.
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Induced optical activity of 2,4-dinitrophenyl-lysine specifically bound to mouse MOPC-315 myeloma protein.与小鼠MOPC-315骨髓瘤蛋白特异性结合的2,4-二硝基苯基赖氨酸的诱导旋光性。
Nature. 1971 Jul 16;232(5307):192-4. doi: 10.1038/232192a0.
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