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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Cooke R D, Holbrook J J

Biochem J. 1974 Jul;141(1):71-8. doi: 10.1042/bj1410071.

A continuous fluorimetric assay for blood-clotting Factor XIII based on the incorporation of dansylcadaverine into casein was investigated. 2. Hammarsten casein was fractionated to yield the beta-casein, which was dephosphorylated and acetylated to give a substrate which itself did not bind calcium and which was not cross-linked by the activated Factor. 3. The modified beta-casein was used as substrate for a continuous fluorimetric assay and as substrate for the incorporation of radioactive glycine ethylester. 4. A linked fluorimetric assay for the zymogen is described. 5. The K(m) for calcium was redetermined and at 0.2mm was in the physiological range and much lower than the values reported by others using substrates which interact with calcium. 6. The K(m) for casein is about 14mum.

研究了一种基于将丹磺酰尸胺掺入酪蛋白的连续荧光法测定血液凝固因子XIII。2. 将哈马斯坦酪蛋白分级分离得到β-酪蛋白，对其进行去磷酸化和乙酰化处理，得到一种自身不结合钙且不会被活化因子交联的底物。3. 修饰后的β-酪蛋白用作连续荧光法测定的底物以及掺入放射性甘氨酸乙酯的底物。4. 描述了一种针对该酶原的偶联荧光法测定。5. 重新测定了钙的米氏常数（K(m)），其在0.2mmol/L时处于生理范围内，且远低于其他使用与钙相互作用底物的人所报道的值。6. 酪蛋白的米氏常数约为14μmol/L。

Cooke R D, Holbrook J J

Biochem J. 1974 Jul;141(1):71-8. doi: 10.1042/bj1410071.

A continuous fluorimetric assay for blood-clotting Factor XIII based on the incorporation of dansylcadaverine into casein was investigated. 2. Hammarsten casein was fractionated to yield the beta-casein, which was dephosphorylated and acetylated to give a substrate which itself did not bind calcium and which was not cross-linked by the activated Factor. 3. The modified beta-casein was used as substrate for a continuous fluorimetric assay and as substrate for the incorporation of radioactive glycine ethylester. 4. A linked fluorimetric assay for the zymogen is described. 5. The K(m) for calcium was redetermined and at 0.2mm was in the physiological range and much lower than the values reported by others using substrates which interact with calcium. 6. The K(m) for casein is about 14mum.

研究了一种基于将丹磺酰尸胺掺入酪蛋白的连续荧光法测定血液凝固因子XIII。2. 将哈马斯坦酪蛋白分级分离得到β-酪蛋白，对其进行去磷酸化和乙酰化处理，得到一种自身不结合钙且不会被活化因子交联的底物。3. 修饰后的β-酪蛋白用作连续荧光法测定的底物以及掺入放射性甘氨酸乙酯的底物。4. 描述了一种针对该酶原的偶联荧光法测定。5. 重新测定了钙的米氏常数（K(m)），其在0.2mmol/L时处于生理范围内，且远低于其他使用与钙相互作用底物的人所报道的值。6. 酪蛋白的米氏常数约为14μmol/L。