Cox R B, Zatman L J
Biochem J. 1974 Aug;141(2):605-8. doi: 10.1042/bj1410605.
Hexose phosphate synthase and hexulose phosphate isomerase activities were found in trimethylamine-grown bacterium 2B2, a facultative methylotroph. The activities were separated by column chromatography of cell extracts on DEAE-cellulose. Hexulose phosphate isomerase activity was measured spectrophotometrically by using the product of the hexose phosphate synthase reaction as substrate.
在兼性甲基营养菌三甲胺生长菌2B2中发现了磷酸己糖合酶和磷酸己酮糖异构酶的活性。通过在DEAE-纤维素上对细胞提取物进行柱色谱分离这些活性。以磷酸己糖合酶反应的产物为底物,用分光光度法测定磷酸己酮糖异构酶的活性。